Epigentek

Chromoplasts Chromatin Extraction Kit

Background Knowledge
Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space under quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, a ChIP methodology with explicit antibodies straight in the direction of quite a few transcriptional parts is broadly in demand.

Principle & Course of
The ChromaFlash Plant Chromatin Extraction Bundle accommodates the entire reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

Background Knowledge

Chromatin immunoprecipitaton (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space under quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, the ChIP methodology with explicit antibodies straight in the direction of quite a few transcriptional parts is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Bundle is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this package deal will be utilized in numerous chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Bundle. The isolated chromatin can even be utilized in several chromatin-related capabilities equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Terribly fast course of: your whole course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
  • Useful and versatile: the package deal is acceptable for preparing every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.
epigentek chromoplasts chromatin
epigentek chromoplasts chromatin

 

Principle & Course of
The ChromaFlash Chromatin Extraction Bundle accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, up to date and frozen tissues, and so forth. The amount of cells and tissues for each preparation might be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues.  Yield of chromatin is roughly 4 ug per 106  cells or per 50 mg tissues.

ExoDNA? Extraction Kit

K1242-25
EUR 705.6

ExoDNA? Extraction Kit

K1242-50
EUR 1057.2

miRNA Extraction Kit

K1456-50 50 Preps
EUR 661.2

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Total Protein Extraction Kit

BSP003 50Preps
EUR 130.99

Plant Protein Extraction Kit

BSP004 20Preps
EUR 133.08

Nuclear Protein Extraction Kit

BSP009 50Preps
EUR 106.98

FFPE Protein Extraction Kit

BSP058 50Preps
EUR 122.64

Membrane Protein Extraction Kit

55R-1357 50 assays
EUR 888
Description: Membrane Protein Extraction Kit for use in the research laboratory

Mammalian Cell Extraction Kit

55R-1358 500 assays
EUR 457.2
Description: Mammalian Cell Extraction Kit for use in the research laboratory

Quick Gel Extraction Kit

20-abx098085
  • EUR 343.20
  • EUR 243.60
  • 200 rxns
  • 50 rxns

Up RNA Extraction Kit

20-abx098087
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

Plant RNA extraction Kit

20-abx098088
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

Exosomal RNA Extraction Kit

P200R,-part-of-P200 - Ask for price

Exosomal DNA Extraction Kit

P230-25 - Ask for price

Exosomal DNA Extraction Kit

P230-50 - Ask for price

Plant Tissue Extraction Kit

K296-50
EUR 548.4

Total Protein Extraction Kit

K3011010 1 kit
EUR 444
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein Extraction Kit

K268-50
EUR 646.8

Mammalian Cell Extraction Kit

K269-500
EUR 352.8

Membrane Protein Extraction Kit

K2113-50 50 assays
EUR 652.8

Mammalian Cell Extraction Kit

K2114-500 500 assays
EUR 385.2

Bacterial RNA Extraction Kit

R403-01 100 rxn
EUR 186

EpiQuik Nuclear Extraction Kit 

OP-0002-1 100 Extractions
EUR 211.2

EpiQuik Nuclear Extraction Kit 

OP-0002
  • EUR 350.58
  • EUR 211.20
  • 100 Assays
  • 100 Extractions

Membrane Protein Extraction Kit

MPE01-12S 0.5 ml
EUR 117.02
Description: This product includes 0.5 ml of 12 solutions for screening and micro-scale preparative extraction of membrane proteins (up to 1.5 mg) from the cell membranes.

Column Packing Kit

PACK-KIT 1pack
EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

EpiQuik Chromatin Accessibility Assay Kit 

P-1047-48 48 Reactions
EUR 611.6

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit 

P-2002-1 24 Reactions
EUR 312.4

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit 

P-2002-2 48 Reactions
EUR 500.5

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit 

P-2002-3 96 Reactions
EUR 738.1

ChromaFlash Chromatin Isolation & Shearing Kit 

P-2023-100 100 Preparations
EUR 189.2

EpiQuik Chromatin Accessibility Assay Kit 

P-1047
  • EUR 910.86
  • EUR 611.60
  • 48 Assays
  • 48 Reactions

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit 

P-2002
  • EUR 1084.86
  • EUR 312.40
  • EUR 500.50
  • EUR 738.10
  • 96 Reactions
  • 24 Reactions
  • 48 Reactions
  • 96 Reactions

ChromaFlash Chromatin Isolation & Shearing Kit 

P-2023
  • EUR 324.48
  • EUR 189.20
  • 100 Preperations
  • 100 Preparations

Triton Extraction Buffer(TEB), for Histon Extraction

T8300-100 100ml
EUR 151.2

Triton Extraction Buffer(TEB), for Histon Extraction

T8300-500 500ml
EUR 170.4

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