
Chromoplasts Chromatin Extraction Kit
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Background Knowledge
Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space under quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, a ChIP methodology with explicit antibodies straight in the direction of quite a few transcriptional parts is broadly in demand.
Background Knowledge
Chromatin immunoprecipitaton (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space under quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, the ChIP methodology with explicit antibodies straight in the direction of quite a few transcriptional parts is broadly demanded.
Product Overview
The ChromaFlash Chromatin Extraction Bundle is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this package deal will be utilized in numerous chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Bundle. The isolated chromatin can even be utilized in several chromatin-related capabilities equal to in vitro protein-DNA binding assays and nuclear enzyme assays.
- Terribly fast course of: your whole course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
- Useful and versatile: the package deal is acceptable for preparing every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
- Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.

Principle & Course of
The ChromaFlash Chromatin Extraction Bundle accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.
Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, up to date and frozen tissues, and so forth. The amount of cells and tissues for each preparation might be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues. Yield of chromatin is roughly 4 ug per 106 cells or per 50 mg tissues.
miRNA Extraction Kit |
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K1456-50 | Biovision | 50 Preps | EUR 661.2 |
ExoDNA? Extraction Kit |
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K1242-25 | Biovision | each | EUR 705.6 |
ExoDNA? Extraction Kit |
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K1242-50 | Biovision | each | EUR 1057.2 |
Total Protein Extraction Kit |
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BSP003 | Bio Basic | 50Preps | EUR 130.99 |
Plant Protein Extraction Kit |
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BSP004 | Bio Basic | 20Preps | EUR 133.08 |
Nuclear Protein Extraction Kit |
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BSP009 | Bio Basic | 50Preps | EUR 106.98 |
FFPE Protein Extraction Kit |
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BSP058 | Bio Basic | 50Preps | EUR 122.64 |
Membrane Protein Extraction Kit |
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55R-1357 | Fitzgerald | 50 assays | EUR 888 |
Description: Membrane Protein Extraction Kit for use in the research laboratory |
Mammalian Cell Extraction Kit |
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55R-1358 | Fitzgerald | 500 assays | EUR 457.2 |
Description: Mammalian Cell Extraction Kit for use in the research laboratory |
Quick Gel Extraction Kit |
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20-abx098085 | Abbexa |
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Up RNA Extraction Kit |
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20-abx098087 | Abbexa |
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Plant RNA extraction Kit |
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20-abx098088 | Abbexa |
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Total Protein Extraction Kit |
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K3011010 | Biochain | 1 kit | EUR 444 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Membrane Protein Extraction Kit |
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K2113-50 | ApexBio | 50 assays | EUR 652.8 |
Mammalian Cell Extraction Kit |
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K2114-500 | ApexBio | 500 assays | EUR 385.2 |
Bacterial RNA Extraction Kit |
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R403-01 | Vazyme | 100 rxn | EUR 186 |
EpiQuik Nuclear Extraction Kit |
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OP-0002-1 | EpiGentek | 100 Extractions | EUR 211.2 |
EpiQuik Nuclear Extraction Kit |
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OP-0002 | EpiGentek |
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Membrane Protein Extraction Kit |
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MPE01-12S | ProFoldin | 0.5 ml | EUR 117.02 |
Description: This product includes 0.5 ml of 12 solutions for screening and micro-scale preparative extraction of membrane proteins (up to 1.5 mg) from the cell membranes. |
Membrane Protein Extraction Kit |
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K268-50 | Biovision | each | EUR 646.8 |
Mammalian Cell Extraction Kit |
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K269-500 | Biovision | each | EUR 352.8 |
Plant Tissue Extraction Kit |
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K296-50 | Biovision | each | EUR 548.4 |
Frit Kit |
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FRIT-KIT | Next Advance | 1each | EUR 148.8 |
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
Column Packing Kit |
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PACK-KIT | Next Advance | 1pack | EUR 1242 |
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
EpiQuik Chromatin Accessibility Assay Kit |
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P-1047-48 | EpiGentek | 48 Reactions | EUR 611.6 |
EpiQuik Chromatin Immunoprecipitation (ChIP) Kit |
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P-2002-1 | EpiGentek | 24 Reactions | EUR 312.4 |
EpiQuik Chromatin Immunoprecipitation (ChIP) Kit |
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P-2002-2 | EpiGentek | 48 Reactions | EUR 500.5 |
EpiQuik Chromatin Immunoprecipitation (ChIP) Kit |
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P-2002-3 | EpiGentek | 96 Reactions | EUR 738.1 |
ChromaFlash Chromatin Isolation & Shearing Kit |
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P-2023-100 | EpiGentek | 100 Preparations | EUR 189.2 |
EpiQuik Chromatin Accessibility Assay Kit |
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P-1047 | EpiGentek |
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EpiQuik Chromatin Immunoprecipitation (ChIP) Kit |
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P-2002 | EpiGentek |
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ChromaFlash Chromatin Isolation & Shearing Kit |
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P-2023 | EpiGentek |
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PCR Mycoplasma Detection Kit |
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M034-Kit | TOKU-E | Kit | EUR 319.2 |
Triton Extraction Buffer(TEB), for Histon Extraction |
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T8300-100 | GenDepot | 100ml | EUR 151.2 |
Triton Extraction Buffer(TEB), for Histon Extraction |
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T8300-500 | GenDepot | 500ml | EUR 170.4 |
DNA Extraction Kit in Tubes |
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D100T | Cygnus Technologies | 1 kit (50 tubes) | EUR 562.8 |
Description: DNA Extraction Kit in Tubes by Cygnus Technologies is available in Europe via Gentaur. |
DNA Extraction Kit in Wells |
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D100W | Cygnus Technologies | 1 kit (96 wells plate) | EUR 562.8 |
Description: DNA Extraction Kit in Wells by Cygnus Technologies is available in Europe via Gentaur. |
Inclusion Body Protein Extraction Kit |
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BS686 | Bio Basic | 1kit, 10prep | EUR 127.86 |