Apexbio cell counting kit-8

Description

Our product Cell Counting Package deal-8 (CCK-8) current a further useful and delicate approach for the evaluation of cell amount dedication and cell proliferationtotoxicity assay than earlier strategies. The tools makes use of a extraordinarily water-soluble tetrazolium salt, WST-8, which produces a water-soluble formazan dye upon low cost inside the presence of an electron mediator. The amount of the formazan generated by dehydrogenases is straight in proportion to the numbers of residing cells. The detection sensitivity by CCK-Eight is bigger than completely different tetrazolium salts resembling MTT, XTT, MTS or WST-1.

Choices

Further Delicate than MTT, MTS, or WST-1

No Toxicity to Cell

Simpler Steps, No pure solvents required

Regular One Bottle Reply, ready-to-use

Background

Cell proliferation shall be detected by numerous methods. Cell proliferation induces an increase inside the train of the mitochondrial dehydrogenases, which cleaves the tetrazolium salt to formazan.

The WST Cell Proliferation Colorimetric Assay Package deal plus provides the most effective and most delicate technique for quantification of cell proliferation and viability. Cell proliferation causes the rise inside the amount of formazan dye long-established that is straight proportional to the number of residing cells and shall be quantified by measuring the absorbance of the dye decision at 440 nm using microtiter plate reader. The tools might be utilized for the analysis of cell proliferation in response to pharmaceutical compounds, cytotoxic compounds like anticancer drugs and plenty of completely different toxic brokers.

The assay may additionally be used for the detection of cell proliferation in response to cytokines, mitogens, progress components and nutritional vitamins, and so forth. The assay might be very easy and straightforward, merely add-and-read, requiring no solubilization, no washing and no harvesting steps, and is regular, sooner and further delicate than MTS, MTT or XTT-based assays. Your total assay shall be carried out in a microtiter plate and correlates properly with the [³H]-thymidine incorporation assay.

Choices & Properties

Choices	Fast and useful Simple one-step course of; takes decrease than 4 hours. Makes use of a water-soluble tetrazolium salt WST reagent. The technique is very easy that requires no washing/harvesting/or solubilization steps and is faster and further delicate than MTT/XTT/or MTS-based assays. Your total assay shall be carried out straight in a 96-well plate.
Supply	Gel pack
Storage Circumstances	Retailer at -20°C.

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2. Xiangli Zhuang, Bo Wu, et al. “Influence of Xingbi Gel Nasal Drops on Fyn-STAT5 Pathway in Nasal Mucosa Fibroblasts of Guinea Pigs with Allergic Rhinitis.” Evid Based Complement Alternat Med. 2021 Mar 20;2021:6686815. PMID:33824677
3. Rui-Qi Wang, Xiao-Ran Prolonged, et al. “Lnc-GAN1 expression is said to good survival and suppresses tumor growth by sponging mir-26a-5p to activate PTEN signaling in non-small cell lung most cancers.” J Exp Clin Most cancers Res. 2021 Jan 6;40(1):9. PMID:33407724
4. Peng Zhao, Jianhua Cheng, et al. “LncRNA PCAT6 regulates the event of pituitary adenomas by regulating the miR-139-3p/BRD4 axis.” Most cancers Cell Int. 2021 Jan 6;21(1):14. PMID:33407504
5. John Culver Taylor, Xinsheng Gao, et al. “A form VII secretion system of Streptococcus gallolyticus subsp. gallolyticus contributes to gut colonization and the occasion of colon tumors.” PLoS Pathog. 2021 Jan 6;17(1):e1009182. PMID:33406160
6. Guangyu Ji, Wenjuan Zhou, et al. “Melatonin inhibits proliferation and viability and promotes apoptosis in colorectal most cancers cells by the use of upregulation of the microRNA-34a/449a cluster.” Mol Med Rep. 2021 Mar;23(3):187. PMID:33398374

Cell Counting Kit-8 (CCK-8)
K1018-5	ApexBio	1x5 ml (500 tests)	EUR 142.8

Cell Counting Kit-8
HY-K0301	MedChemExpress	120 mL (12000 T)	EUR 1362

Cell Counting Kit (CCK)
20-abx098245	Abbexa	EUR 627.60 EUR 493.20 EUR 861.60 EUR 560.40	10 ml 1 ml 30 ml 5 ml

CCK-8 Cell Counting Kit
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CCK-8 Cell Counting Kit
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Cell Counting Kit-8 (CCK-8)
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Cell Counting Kit-8 (CCK-8)
B34302	Bimake	5 mL (500 ractions)	EUR 156
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK-8)
B34304	Bimake	25 mL (2500 ractions)	EUR 378
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK8) Cell Viability Assay Kit
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Cell Counting Pot Square Base
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Counting Slide 2 Cell Mcmaster
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Plastic cell counting slides Kovas Slide
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CORNING COUNTING CHAMBER FOR CORNING CELL COUNTER
480200	CORNING	1/pk	EUR 170.4
Description: Lab Equipment; Cell Counters

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K511-2500	Biovision	each	EUR 744

Bacterial Counting Colorimetric Assay Kit
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Coverslip for counting chamber
Z5000	Scientific Laboratory Supplies	PK10	EUR 24.15

Counting Chamber Helber Bacteria
COU9100	Scientific Laboratory Supplies	EACH	EUR 139.7

Fast Read Disposable Counting Chambers
390497	Scientific Laboratory Supplies	PK100	EUR 195.6

BRAND(TM) BLAUBRAND(R) COUNTING CHAMBER
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Counting Chamber Improved Neubauer 2 x Bright
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Synbiosis AutoCOL Fully Automated Colony Counting System
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ApexBlue? Quick Cell Viability Fluorometric Assay Kit
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HIF-1 Alpha Cell Based ELISA Kit
CBA-281	Cell Biolabs	96 assays	EUR 734.4
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.

CytoSelect BrdU Cell Proliferation ELISA Kit
CBA-251	Cell Biolabs	96 assays	EUR 637.2
Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection). Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.

CytoSelect 48-Well Cell Contraction Assay Kit
CBA-5021	Cell Biolabs	48 assays	EUR 762
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A5001-300	ApexBio	300 tests	EUR 338.4

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CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit
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CytoSelect Clonogenic Tumor Cell Isolation Kit (5 x 5 preps)
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Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

CytoSelect 24-Well Cell Contraction Assay Kit (Floating Matrix Model)
CBA-5020	Cell Biolabs	24 assays	EUR 679.2
Description: Cell Biolabs? Cell Contraction Assays (Floating Matrix Model) provide a simple, in vitro system to assess cell contractivity and screen cell contraction mediators. The proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction.

293AD Cell Line
AD-100	Cell Biolabs	1 vial	EUR 553.2
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

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AAV-100	Cell Biolabs	1 vial	EUR 609.6
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

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