BioRag Bio Resource for Array Genes

Lipid peroxidation assay protocol

• Kelly
• December 4, 2021
• 0

Lipid peroxidation refers again to the oxidative degradation of lipids. On this course of free radicals take electrons from the lipids (usually in cell membranes), resulting in cell hurt. Quantification of lipid peroxidation is essential to judge oxidative stress. Lipid peroxidation varieties reactive aldehydes similar to malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as pure bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are typically used as markers of lipid peroxidation, and to assay for oxidative hurt / oxidative stress.

Lipid peroxidation assay protocol summary:

– add TBA reply to samples and necessities, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min
– swap to wells of microplate
– analyze with microplate reader
For higher sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet sooner than analysis.

Lipid Peroxidation (MDA + HNE) Assay Bundle

Lipid peroxidation is a extensively identified occasion of oxidative hurt in cell membranes, lipoproteins, and totally different lipid-containing buildings. Peroxidative modification of unsaturated phospholipids, glycolipids, and ldl ldl cholesterol can occur in a number of reactions. They’re usually triggered by i) free radical species similar to oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated low cost of hydrogen peroxide or ii) non-radical species similar to singlet oxygen, ozone, and peroxynitrite generated by the response of superoxide with nitric oxide.

Malondialdehyde (MDA) and 4-hydroxyalkenals are important toxic byproducts of lipid peroxidation. So, the measurement of the portions of such aldehydes corresponds to an index of lipid peroxidation in vitro and in vivo. 4-Hydroxynonenal (4-HNE) is a severe product of the peroxidative decomposition of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic, mutagenic, and genotoxic properties. Furthermore, elevated ranges of HNE had been current in plasma and diverse organs beneath oxidative stress circumstances. The reality is, MDA is in numerous conditions most likely probably the most ample specific individual aldehyde ensuing from lipid peroxidation. In vitro MDA can alter proteins, DNA, RNA, and loads of totally different biomolecules.

Bioquochem’s LPO assay gear measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed assault on the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). Due to this, this response offers a diindolylalkane (chromophore) with most absorbance inside the space of 580-620 nm.

In our assay an indol (Reagent A) reacts quickly with MDA and HNE in acidic medium, yielding a chromophore (C) with a extreme molar extinction coefficient at its maximal absorption wavelength of 586 nm.

 

 

• Product overview

  Lipid Peroxidation (MDA) Assay Bundle (Colorimetric/Fluorometric) (ab118970) provides a helpful software program for delicate detection of malondialdehyde (MDA).

  Inside the lipid peroxidation assay protocol, the MDA inside the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct might be merely quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA ranges as little as 1 nmol/successfully colorimetrically and 0.1 nmol/successfully fluorometrically.

  The MDA assay can be refered to as a TBARS assay.

  Chinese language language protocol on the market. See protocols half beneath.

  For an alternate MDA assay, with out the heating steps required inside the TBARS assay, try MDA assay ab233471.

• Notes

   

  Related merchandise

  Evaluation the oxidative stress marker and assay info, or the whole metabolism assay info to check additional assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and as well as how one can assay metabolic function in dwell cells using your plate reader.

  Moreover see the favored 4-HNE Assay Bundle ab238538 instead marker of lipid peroxidation and oxidative stress.

  
  How totally different researchers have used Lipid Peroxidation Assay Bundle ab118970

  The MDA/TBARs assay gear has been utilized in publications in a variety of sample types, along with:
  – Human: serum¹, hippocampal principal cell extracts², A375 cultured cell lysates³, plasma and platelet samples⁴
  – Mouse: neuronal cell lysates⁵, coronary coronary heart tissue extract⁶, plasma⁷, cell extracts⁸
  – Rat: hippocampal tissue extracts⁹, cardiomyocyte extracts of cultured cells¹⁰, lung lysates¹¹
  – Pig: serum¹²

  ^{References: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018,  4 – Mustafa AG et al. 2018, 5 – Murphy Okay et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE and Kang KS 2019}

• Platform

  Microplate reader

Lipid peroxidation Assay Kit
SH0312-3	Jiaxing Korain Biotech Ltd (BT Labs)	100T	EUR 880

Lipid peroxidation inhibitor 1
HY-U00319	MedChemExpress	1mg	EUR 1448.4

Lipid peroxidation inhibitor 1
T10166-10mg	TargetMol Chemicals	10mg	Ask for price
Description: Lipid peroxidation inhibitor 1

Lipid peroxidation inhibitor 1
T10166-1g	TargetMol Chemicals	1g	Ask for price
Description: Lipid peroxidation inhibitor 1

Lipid peroxidation inhibitor 1
T10166-1mg	TargetMol Chemicals	1mg	Ask for price
Description: Lipid peroxidation inhibitor 1

Lipid peroxidation inhibitor 1
T10166-50mg	TargetMol Chemicals	50mg	Ask for price
Description: Lipid peroxidation inhibitor 1

Lipid peroxidation inhibitor 1
T10166-5mg	TargetMol Chemicals	5mg	Ask for price
Description: Lipid peroxidation inhibitor 1

Lipid peroxidation inhibitor 1
MBS5765948-5mg	MyBiosource	5mg	EUR 915

Lipid peroxidation inhibitor 1
MBS5765948-5x5mg	MyBiosource	5x5mg	EUR 3970

Lipid Peroxidation (MDA) Assay Kit
abx096010-100Assays	Abbexa	100 Assays	EUR 453.6

Lipid Peroxidation MDA Assay Kit
EGQA0070	EnoGene	100T	EUR 85

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K2167-500	ApexBio	500T	EUR 198.4
Description: Cell Biology|RNS detection

Lipid Peroxidation (MDA) Assay Kit
GWB-AXR340	GenWay Biotech	100 assays	Ask for price

Lipid Peroxidation (LPO) Assay Kit
abx096010-100l	Abbexa	100 µl	EUR 425

Lipid Peroxidation (LPO) Assay Kit
abx096010-1ml	Abbexa	1 ml	Ask for price

Lipid Peroxidation (LPO) Assay Kit
abx096010-200l	Abbexa	200 µl	Ask for price

Lipid Peroxidation (LPO)Assay Kit
BC015-50T	ELK Biotech	50T	EUR 200

Lipid Peroxidation (MDA) Assay kit
MBS9718963-48Tests	MyBiosource	48Tests	EUR 160

Lipid Peroxidation (MDA) Assay kit
MBS9718963-5x96Tests	MyBiosource	5x96Tests	EUR 745

Lipid Peroxidation (MDA) Assay kit
MBS9718963-96Tests	MyBiosource	96Tests	EUR 170

Lipid Peroxidation Colorimetric Assay Kit
K454-100	Biovision	each	EUR 652.8

Human Lipid Peroxidation (LPO) Elisa Kit
EK710559	AFG Bioscience LLC	96 Wells	EUR 0.1

Porcine Lipid Peroxidation (LPO) ELISA Kit
SL0272Po	Sunlong	-	Ask for price

Lipid Peroxidation Assay, Catalog: MA-0102
MA-0102	Akrivis Bio	100 wells	EUR 525

Rat Lipid Peroxidation ELISA Kit = Rat Lipid Peroxide(LPO)ELISA Kit
SL1340Ra	Sunlong	-	Ask for price

Lipid peroxidation assay kit (Colorimetric method)
MBS2540553-48Test	MyBiosource	48Test	EUR 325

Lipid peroxidation assay kit (Colorimetric method)
MBS2540553-48Tests	MyBiosource	48Tests	EUR 325

Lipid peroxidation assay kit (Colorimetric method)
MBS2540553-5x96Tests	MyBiosource	5x96Tests	EUR 1900

Lipid peroxidation assay kit (Colorimetric method)
MBS2540553-96Test	MyBiosource	96Test	EUR 415

Lipid peroxidation assay kit (Colorimetric method)
MBS2540553-96Tests	MyBiosource	96Tests	EUR 415

Bodi Fluorâ„¢ 581/591 C11 *Lipid Peroxidation Probe*
23290	AAT Bioquest	1mg	EUR 199
Description: Bodi Fluorâ„¢ 581/591 C11 is the same molecule as Bodipy 581/591 C11.

CheKine™ Micro Lipid Peroxidation (MDA) Assay kit
KTB1050-each	Abbkine	each	Ask for price

CheKine™ Micro Lipid Peroxidation (MDA) Assay kit
KTB1050-48T	Abbkine	48 T	EUR 39
Description: Abbkine CheKine™ Micro Lipid Peroxidation (MDA) Assay kit is designed for the quantitative determination of lipid peroxidation.

CheKine™ Micro Lipid Peroxidation (MDA) Assay kit
KTB1050-96T	Abbkine	96 T	EUR 49
Description: Abbkine CheKine™ Micro Lipid Peroxidation (MDA) Assay kit is designed for the quantitative determination of lipid peroxidation.