Gram Stain Kit
Qualification: for microscopy
Quality level: 100
Agency: according to GB 4789.30-2016
Product line: biochemistry
Duration: limited shelf life, the expiration date on the label
Technique(s): microbe identification | staining: Adequate
The spectrum of antibiotic activity
- Gram-negative bacteria
- Gram-positive bacteria
- clinic tests
- food and drinks
The Gram stain kit is used in the differential staining technique that is most widely applied in all microbiology discipline laboratories. It is one of the most important criteria in any identification scheme for all types of bacterial isolates. There are many physiological differences between gram-positive and gram-negative cell walls. Since Christian Gram discovered the Gram stain, this process has been extensively investigated and redefined.
In practice, a thin smear of bacterial cells is stained with crystal violet and then treated with iodine-containing mordant to increase the binding of the primary stain. A destaining solution of alcohol or acetone is used to remove crystal violet from loosely binding cells and then safranin is used as a counterstain to provide colour contrast in those cells that are disdained. On a properly stained smear using the Gram stain procedure, gram-positive bacteria appear blue to purple, and gram-negative cells appear pink to red.
The Gram stain method is one of the most important staining techniques in microbiology. It is also the most important criterion in any identification scheme for all types of bacterial isolates to differentiate Gram-positive and Gram-negative bacteria.
- The kit contains (250 ml each):
- 94448 Gram’s crystal violet solution
- 90107 Gram’s Iodine Solution
- 75482 Gram’s staining solution
- 94635 Gram’s Safranin Solution