EasyNAT Canine and Feline TOX Nucleic Acid Test


The Rapid Canine Brucella Ab Test Kit is a chromatographic immunoassay for the qualitative detection of antibodies to Brucella canis in canine whole blood, plasma or serum. The Rapid Canine Brucella Ab Test Kit has two letters which are the test line (T) and control line (C) on the surface of the device. The test line and control line in the result window is not visible before any sample is applied.

The control line is a reference line that indicates that the test is performing correctly. The control line has to appear each time the test is performed. If there are antibodies to Brucella canis in the sample, a purple test line will appear in the result window. Highly selective Brucella canis antigens are used as capture and detector in the assay. These antigens are capable of detecting antibodies against Brucella canis directly, with great precision.

Storage and Stability

  • Store the test kit at 2~30℃. DO NOT FREEZE.
  • Do not store the test kit in direct sunlight.
  • The test kit is stable within the expiration date marked on the package label.

Test Limitations

  • Although the Anigen Rapid C. Brucella Ab Test Kit is highly accurate in detecting antibodies to Brucella canis, a low incidence of false results may occur. Other clinical tests are required if questionable results are obtained.
  • As with all diagnostic tests, a definitive clinical diagnosis should not be based on the results of a single test, but should only be made by the veterinarian after all clinical and laboratory findings have been evaluated.
  • The reading window may display a light pink background colouration; this will not affect the accuracy of the results.
  • The manufacturer and its distributors are not responsible for the consequences of misuse or misinterpretation of the results provided by the test.

Sample Collection and Preparation

Canine whole blood, serum or plasma should be used with this test.

  • [Whole Blood] Collect whole blood in the anticoagulant tube (max. vol. 1.5 mL) provided. If the anticoagulated whole blood is not tested immediately, it should be refrigerated at 2~8°C and used within 24 hours.
  • [Serum] Collect all the blood in the collection tube (not containing anticoagulants such as heparin, EDTA, and sodium citrate), allow it to stand for 30 minutes for blood clotting, and then centrifuge to obtain the serum supernatant.
  • [Plasma] Collect all the blood in the collection tube (containing anticoagulants such as heparin, EDTA, and sodium citrate), and then centrifuged to obtain plasma.

If serum or plasma samples are not to be tested immediately, they should be refrigerated at 2~8°C. For longer storage, freezing is recommended. Frozen samples should be brought to room temperature (15~30°C) before use. Samples containing precipitates may give inconsistent test results. Such samples must be cleared prior to testing. The use of hemolytic or bacterially contaminated samples should be avoided. Erroneous results may occur.

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